| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Methanobrevibacter smithii | CAGCTCACAGCCAGCTTCA | AAGTTGACGTCCGGACCA | 60.30 | 58.46 | 229 | 121 |
100.00%
|
100.00%
|
| Primer 82 | Methanobrevibacter smithii | TGCATGCCCAAGAGGTGT | AGGAGGCATTGATCCATCAGT | 59.15 | 58.87 | 208 | 121 |
100.00%
|
100.00%
|
| Primer 83 | Methanobrevibacter smithii | CTGATGTTTCTGGTGATGCGG | AGGAGACACACCTGCAGGA | 59.60 | 60.15 | 194 | 119 |
100.00%
|
100.00%
|
| Primer 84 | Methanobrevibacter smithii | TGATGTTTCTGGTGATGCGG | AGGAGACACACCTGCAGGA | 58.55 | 60.15 | 193 | 119 |
100.00%
|
100.00%
|
| Primer 85 | Methanobrevibacter smithii | AACAGGTCCTGCCCATTGG | GCTGGACAATCACTTCACCC | 59.92 | 58.83 | 158 | 118 |
100.00%
|
50.00%
|
| Primer 86 | Methanobrevibacter smithii | AACAGGTCCTGCCCATTGG | AGCTGGACAATCACTTCACCC | 59.92 | 60.27 | 159 | 118 |
100.00%
|
50.00%
|
| Primer 87 | Methanobrevibacter smithii | TGGAACCGGAGCAACACA | GCAAGCTCGTGAAGAGGCT | 59.09 | 60.38 | 189 | 117 |
100.00%
|
100.00%
|
| Primer 88 | Methanobrevibacter smithii | TGGGTGAAGCTTTCAGGCA | AGACCGCTTCAGCCTGATG | 59.46 | 59.78 | 276 | 116 |
100.00%
|
50.00%
|
| Primer 89 | Methanobrevibacter smithii | ACTTGGGGTGAAATTGATGCA | ACTTCGCCAGCAATTACAGC | 58.39 | 59.20 | 155 | 116 |
100.00%
|
100.00%
|
| Primer 90 | Methanobrevibacter smithii | ACTTGGGGTGAAATTGATGCA | AACTTCGCCAGCAATTACAGC | 58.39 | 59.80 | 156 | 116 |
100.00%
|
100.00%
|